Analytical Services

Analytical Infrastructure

DSK InnoSciences has a State of the Art analytical facility providing world class analytical research services to their clients across the globe. DSK provides its analytical services to those companies who are involved in the manufacture and synthesis of New Chemical Entities (NCEs), Active Pharmaceutical Ingredients, Active Ingredients, Polymers, Carbohydrates, Surfactants, Electronic Materials, Agrochemicals, fine chemicals, Speciality Chemicals etc.

DSK analytical services includes structural elucidation, characterization, raw material analysis, stereochemical analysis, wet chemistry followed by analytical method development, analytical method validation, impurity studies and purification of complex mixtures. Our separation capabilities includes both normal phase and reverse phase chromatographic techniques. DSK also undertakes the analysis of chiral compounds with the help of special HPLC chiral columns and SOR.

Being a ISO 9001:2015 Company, we give importance to quality, documentation, data integrity etc by following various SOPs, guidelines, client requirements and regulatory compliances. Also, DSK undertakes periodic activities such as CMCs, AMCs, calibrations, preventive maintenance and internal audit to ensure smooth functioning of the analytical instruments. This facilitates providing timely service to their clients. We hereby request both industry and academic institutions to utilize our analytical services which would facilitate in speeding up the research process.

Nuclear Magnetic Resonance (NMR)

NMR spectroscopy is one of the most important instruments to identify the molecular structure, composition and dynamics of organic compounds. The same is analyzed by observing and measuring the interaction of nuclear spins when placed in a superconducting magnetic field. DSK’s in-house NMR Spectrometer can perform various experiments as per the following details.
NMR Spectrometer : 400 MHz Jeol Make with 24 sample Autosampler and is equipped with a Multiprobe.
The NMR experiments can be performed in 1D, 2D & 3D depending on the complexity of the system being studied.
1D experiments such as1H, 13C, 19F, 31P, 15N, 39K, 109Ag can be run with this NMR instrument.
2D experiments such as COSY, NOESY, HSQC, HMBC, TOCSY can also be performed with this instrument.

Mass Directed Auto Purification (MDAP) System

MDAP is a combination of both Prep HPLC along with LC-MS system.
Mass-directed preparative chromatography provides higher sensitivity and more specificity than traditional UV-based detection.
Mass detection can easily be 100 times more sensitive than UV and in Scan Mode, the mass detector delivers unambiguous molecular information about every peak.
We can program peak collection based the molecular weight, or the mass to charge ratio – to collect the correct peak of interest.
DSK’single quadrupole Shimadzu LCMS-2020 mass spectrometer along with the Nexera Prep HPLC system utilizes the patented ultrafast (UF) technology, delivers more information about the preparative runs and enables us to collect the right peak.
High yield of fraction collection is achieved by sending only a small amount of sample into the highly sensitive LC/MS using automated proportioning valve.
UF switching, UF scanning and High sensitivity of LCMS-2020 make it an ideal choice for an efficient and reliable fraction of even complex mixtures.
The capacity of purification ranges from 10mg to 2g.

Liquid chromatography - Mass Spectrometry (LC-MS)

LC / MS is an analytical technique that combines the separation power of HPLC with the direct mass measurement of a mass spectrometer as the detector.
HPLC can separate a wide range of compounds, while the mass detector provides valuable information about the molecular weight, structure, identity, quantity, and purity of a given compound.
DSK’ Shimadzu LCMS-2020 Mass Spectrometer along with the Nexera Prep HPLC system can detect compounds in the mass ranges of 50-2000m/z. It has a dual ion source which can continuously perform two types of ionization, ESI and APCI simultaneously.
DSK’ Waters HPLC Alliance with QDaMass Detector can detect compounds in the mass rangesof 50-1250m/z with only ESI as the single ionization source.

High-Performance Liquid Chromatography (HPLC)

HPLC instrument is an analytical chemistry technique applied to separate, identify, and quantify semi- and non-volatile compounds in liquid samples. It is used for the characterization, purification, and quantitation of compounds.
HPLC are used to analyze compounds with chromophore functional groups. The related substance, assay & enantiomeric purity can be studied.
DSK has a few Waters HPLC Alliance with PDA detectors. The operating system pressure is up to 4000psi.
DSK has many Agilent HPLC 1260 Infinity-II series with PDA detector. The operating system pressure is up to 600/800bar.

HPLC with ELSD & RI Detectors

HPLC with Evaporative Light Scattering Detectors (ELSD) are aerosol-based detectors which are used to analyze compounds with non-Chromophore functional groups.
DSK uses an Agilent 1260 Infinity II with PDA & ELSD for such compounds.
HPLC with RI Detector and Gel Permeation Chromatography (GPC) Software are used for those compounds which cannot be deducted by UV detectors such as polymers. The molecular weight ranging from 160 to 500000 g/mol can be identified in these systems.
DSK uses an Agilent 1260 Infinity II with PDA, RI Detector & GPC Software for such polymer compounds.

Gas Chromatography (GC)

Gas chromatography (GC) is an analytical technique for separating and analyzing compounds that can be vaporized without decomposition. Typical uses of GC include testing the purity of a particular substance or separating the different components of a mixture.
DSK uses Agilent 8890 Gas Chromatography (GC) with Flame Ionization Detector (FID) to test those compounds with anon-Chromophore functional group and lowerboiling points.
DSK uses an Agilent 7890B GC with a 7697A Headspace Sampler system to check the Residual Solvents in organic compounds in ppm levels.

Polarimeter

Polarimeter is an instrument used to determine the Optical Rotation and Specific Optical Rotation (SOR) of a compound. The SOR helps in identifying, characterizing and determining the purity of Chiral compounds.
SORareusedto measure the optical activity of a chiral compound and the same is expressed generally in degrees per centimeter per gram per milliliter.
DSK uses a Rudolph Polarimeter Autopol IV Automatic with Model: APIV-6W

Karl-Fischer (KF)

Karl Fischer titration is a classic titration method that uses coulometric or volumetric titration to determine the trace amounts of water in a compound.
DSK uses Lasco KF-Autotitrator.

Lyophilizer

Lyophilizer is an instrument which is used to remove the major portion of water and solvents from a compound via controlled freezing and sublimation. It is also used to dry the compounds for storage.
DSK uses ZirbusLyophilizer for this purpose.

List Of Analytical Services

S.No.	Analytical Techniques	Analysis Type
1	Spectroscopy
	NMR	1D Experiments ¹H, ¹³C, ¹⁹F, ³¹P, ¹⁵N, ³⁹K, ¹⁰⁹Ag 2D Experiments COSY, NOESY, HSQC, HMBC, TOCSY
2	Spectrometric
	LC-MS	Mass Identification & Quantification
3	Separation
	HPLC or UPLC (DAD or PDA)	Qualitative Analysis or Normal Purity Assay Purity Chiral or Enantiomeric Purity Quantitative or Weight Percentage Related Substances or Impurity Profiling
	HPLC (ELSD)	Qualitative Analysis or Normal Purity Assay Purity Quantitative or Weight Percentage
	HPLC (GPC-RI)	Ø Mainly used for Polymer Compounds Ø To identify the amount of polymerization for the molecular weight ranging from 160 to 500000 g/mol
	GC	Qualitative Analysis or Normal Purity Assay Purity Quantitative or Weight Percentage
	GC-HS	Residual solvents analysis as per ICH guidelines
4	Purifications
	Prep HPLC	Purification up to 2 g Mass Directed Auto Purification (MDAP) using LC-MS
5	Wet Analysis
		Specific Optical Rotation (SOR) Melting Point Moisture / Water Content by KF Loss on Drying (LOD) Residue on Ignition 6 hr up to 600 ^OC Lyophilization up to 1200 mL